ABSTRACT
Objective To understand the health literacy of diabetes prevention and control among community residents in Chongqing City, and to provide reference for development of health education and health promotion. Methods In July 2015, a random sampling method was used to select samples of residents aged 18 years and above in Chongqing. The health literacy questionnaire of urban and rural residents was used to investigate. The logistic regression model was used to analyze the factors affecting the health literacy of diabetes. Results The effective 892 respondents were collected from 1000 residents, and the effective rate was 89.20％. There were 440 men and 452 women; the average age was 61.71±12.29; there were 176 residents having diabetes prevention and health literacy, accounting for 19.73％. Multivariate logistic regression analysis showed that female (OR=1.679, 95％CI: 1.028-2.743), culture degree (ORjunior =3.891, 95％CI: 1.658-9.173; ORsecondary and high scho l =2.959, 95％CI:1.374-6.369; ORcol ege and above=5.319, 95％ CI: 2.433-11.628) and the self assessment of health (ORgeneral=2.892, 95％ CI:1.338-6.251) were higher in the diabetes prevention health literacy. The health education methods include medical staff on-site explanation, community lectures, watching video and propaganda columns were more popular. Conclusion The level of health literacy of diabetes was generally low in Chongqing. The community should focus on diabetes health education activities for men and low educated population. The community should carry out more activities about diabetes for residents, which helps to reduce or delay the occurrence of diabetes in the population.
ABSTRACT
Thirst study was purposed to explore the genetic polymorphism of Chinese Zhuang population at HLA-Cw locus by sequence based typing (SBT). A total of 150 unrelated blood samples from Chinese Zhuang population were subjected to sequencing at exon 2, 3 and 4 of HLA-Cw gene in both directions by using SBT technique established by our laboratory. The purified products of sequencing reaction were run by means of electrophoresis on the ABI 3730 DNA Sequencer and the assignment of HLA-Cw genotype was accomplished by using the Assign 3.5 software. The consensus sequence at exon 2, 3 and 4 of HLA-Cw gene for each sample was imported into the Assign 3.5 software. The results showed that 33.33% of tested samples could obtain an unique genotype, genotype in 63.33% of tested samples with ambiguous results could be assigned by ruling out the rare alleles according to the NMDP Rare Allele List File; however, the final genotype in rest 3.33% of the detected samples could be defined when subjected to further confirmatory testing by PCR-SSP. In this detection 16 HLA-Cw alleles were identified, the common alleles with a frequency of > 10% were Cw*0304 > Cw*0102 > Cw*0801 > Cw*0702. The value for gene diversity (GD) was 0.9297, The frequency for Cw*01, 03, 07, 08, 12, 14 (Cw 1 allele group) and Cw*02, 04, 05, 06, 15, 16, 17, 18 (Cw 2 allele group) was 0.8967 and 0.1032, respectively, which indicated that the Cw 1 allele group is the dominant ligand for KIR in Chinese Zhuang population. 51 genotypes were determined and the distribution of genotype frequency was in line with Hardy-Weinberg principle. It is concluded that the obtained HLA-Cw allele frequency and its distribution characteristics of Chinese Zhuang population can provide valuable data in the studies of anthropology and the association of HLA-Cw with disease.